Selective extraction of bacterial DNA from the surfaces of macroalgae.
نویسندگان
چکیده
A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing.
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 75 1 شماره
صفحات -
تاریخ انتشار 2009